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rat anti darpp32  (R&D Systems)


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    Structured Review

    R&D Systems rat anti darpp32
    Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and <t>DARPP32,</t> a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.
    Rat Anti Darpp32, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti darpp32/product/R&D Systems
    Average 93 stars, based on 38 article reviews
    rat anti darpp32 - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "New MiniPromoter Ple389 ( ADORA2A ) drives selective expression in medium spiny neurons in mice and non-human primates"

    Article Title: New MiniPromoter Ple389 ( ADORA2A ) drives selective expression in medium spiny neurons in mice and non-human primates

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-79004-y

    Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and DARPP32, a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.
    Figure Legend Snippet: Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and DARPP32, a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.

    Techniques Used: Injection, Labeling, Marker, Double Immunostaining, Expressing, Recombinant, Virus, Variant Assay

    Source and concentrations of antibodies and probes.
    Figure Legend Snippet: Source and concentrations of antibodies and probes.

    Techniques Used: Concentration Assay, Plasmid Preparation



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    Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and <t>DARPP32,</t> a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.
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    Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and <t>DARPP32,</t> a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.
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    Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and <t>DARPP32,</t> a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.
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    Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and <t>DARPP32,</t> a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.
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    Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and <t>DARPP32,</t> a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.
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    Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and <t>DARPP32,</t> a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.
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    Average 94 stars, based on 1 article reviews
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    R&D Systems darpp32
    Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and <t>DARPP32,</t> a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.
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    Average 94 stars, based on 1 article reviews
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    Image Search Results


    Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and DARPP32, a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.

    Journal: Scientific Reports

    Article Title: New MiniPromoter Ple389 ( ADORA2A ) drives selective expression in medium spiny neurons in mice and non-human primates

    doi: 10.1038/s41598-024-79004-y

    Figure Lengend Snippet: Three MiniPromoters delivered by ICV injection in mice showed co-labeling with a medium spiny neuron-specific marker in the striatum. MiniPromoters, driving EmGFP in rAAV-PHP.B, were delivered by ICV injection into the lateral ventricle of adult mice, and harvested 4 weeks later. High-power view (left) and quantification of co-localization (right) are shown for Ple385 ( GPR6 ), Ple389 ( ADORA2A ), and Ple391 ( DRD2 ) following double immunostaining of striatal cells for GFP and DARPP32, a marker for striatal MSNs. A considerable number of GFP-labelled cells in the striatum co-expressed DARPP32 immunoreactivity after ICV injections of the three MiniPromoters, confirming specific MSNs expression. EmGFP, emerald-green fluorescent protein; rAAV9-PHP.B, recombinant adeno-associated virus packaged in capsid 9 variant PHB-B. Green, anti-GFP; red, anti-DARPP32. Scale bars, 100 µm.

    Article Snippet: Rat anti-DARPP32 , R&D System (Minneapolis, MN) , MAB4230 , Mice (ICV) , 1:1000.

    Techniques: Injection, Labeling, Marker, Double Immunostaining, Expressing, Recombinant, Virus, Variant Assay

    Source and concentrations of antibodies and probes.

    Journal: Scientific Reports

    Article Title: New MiniPromoter Ple389 ( ADORA2A ) drives selective expression in medium spiny neurons in mice and non-human primates

    doi: 10.1038/s41598-024-79004-y

    Figure Lengend Snippet: Source and concentrations of antibodies and probes.

    Article Snippet: Rat anti-DARPP32 , R&D System (Minneapolis, MN) , MAB4230 , Mice (ICV) , 1:1000.

    Techniques: Concentration Assay, Plasmid Preparation